Description
Bacillus coagulans (TBC-025) is a lactic acid-forming bacterial species within the genus Bacillus, is isolated from Soil. Physical appearance of the final product is off-white to creamish white colored powder, the use of Bacillus coagulans in probiotic applications may in improving the vaginal flora, improving abdominal pain and bloating in irritable bowel syndrome patients and increasing immune response to viral challenges. There is evidence from animal research that suggests that bacillus coagulans is effective in both treating as well as preventing recurrence of clostridium difficile associated diarrhea.
Manufacturing
Bacillus coagulans is manufactured through a fermentation process and freeze-dried at a DSIR (Govt. of India) Certified plant.
Morphology
B. coagulans is a Gram-positive rod (0.9 by 3.0 to 5.0 μm in size), catalase positive, spore-forming, motile, and a facultative anaerobe. It may appear Gram-negative when entering the stationary phase of growth. The optimum temperature for growth is 50 °C (122 °F); temperatures tolerated 30-55 °C. IMViC tests VP and MR tests are positive.
Identification
By 16s r RNA sequencing method
Potency
Customized
Storage
In a sealed package store in a cool, dry place, preferably at temperature not exceeding 8°C
Bulk Density
≥0.7 g/ml
Packaging
High barrier foil laminate bags.
Country Of Origin
India
Purity and Legal Status
Local regulations should always be consulted concerning the status of the product as legislation regarding its intended use may vary from country to country.
Microbiological Specifications
- Total Viable Cell: Count : >100 Billion CFU/G
- Yeast and Mold : <100/g
- Enterococci : <100/g
- S. aureus : Absent/g
- E. coli : Absent/Gm
- Salmonella : Absent/Gm
- Coliform : Absent/Gm
Other Specifications
| Test | Specifications |
|---|---|
| Culture Description | Creamish white, lyophilized powder |
| Moisture Content | Less than 5% |
| Morphological Characteristics | Bacillus coagulans is gram-positive rod (0.9 by 3.0 to 5.0 μm in size) |
| Bulk Density | ≥ 0.7 g/ml |
| Particle Size | ≥ 90% < 250 µm |
| Heavy metals (Pb) | ≤ 1ppm |
| Heavy metals (As) | ≤ 2ppm |
| Total Heavy Metals (Ar, Cd, Pb, Cu and Hg) | ≤ 10 ppm |
| Smell (Olfactory) | Sweet, fermented Smell |
Annexure I
FASTA Sequence:
TGGAGAGTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCCTAATACATGCAAGTCGTGCGGACCTTTTAAAAGCTTGCTTTTAAAAGGTTAGCGGCGGACGGGTGAGTAACACGTGGGCAACCTGCCTGTAAGACTGGGATAACCCGGGAAACCGGGGCTAATACCRGATAGTTTTTTCCTCCGCATGGAGGAAAAAGGAAAGGCGGCTTCGGCTGCCACTTACAGATGGGCCCGCGGCGCATTAGCTAGTTGGCGGGGTAACRGCCCACCAAGGCAACGATGCGTAGCCGACCTGAGAGGGTGATCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGACGAAAGTCTGACGGAGCAACGCCGCGTGAGTGAAGAAGGCCTTCGGGTCGTAAAACTCTGTTGCCGGGGAAGAACAAGTGCCGTTCGAACAGGGCGGCGCCTTGACGGTACCCGGCCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGCGGCTTCTTAAGTCTGATGTGAAATCTTG
BLAST result (Sequences producing significant alignments with NCBI Genomic data)
